Fig. 2

Effects of rSjCa8 on the apoptosis of RAW264.7 cells. RAW264.7 cells were treated with PBS, an apoptosis inducer (positive control), 20 μg/ml rSj13, or 1 and 20 μg/ml rSjCa8 for 24 or 48 h. Annexin-V-FITC-PI staining allows live, apoptotic, and dead cells to be identified using a flow cytometer. The x-axis indicates the Annexin V-positive cells, and the y-axis displays PI-positive cells. This experiment was performed in triplicate