Table 4 TOSV isolation and RNA detection in sand fly processed as pools
Sandflies collection and storage methods | Day post infection | TOSV isolation | RNA detection | |
|---|---|---|---|---|
TOSV RTqPCR | Nested-RT-PCR for SFNV complex of Phlebovirus | |||
Pos/tested (%) | Pos/tested (%) mean ± SD | Pos/tested (%) | ||
AS-CDC | 0 | 5/5 (100) | 5/5 (100) 3.17 ± 0.22 | 3/3 (100) |
DS-CDC | 1 | 5/5 (100) | 5/5 (100) 2.95 ± 0.28 | 3/3 (100) |
DS-St | 1 | 4/5 (80) | 5/5 (100) 3.04 ± 0.18 | 4/4 (100) |
2 | 2/5 (40) | 5/5 (100) 2.97 ± 0.39 | 4/4 (100) | |
3 | 1/5 (20) | 5/5 (100) 3.14 ± 0.28 | 3/3 (100) | |
4 | 2/5 (40) | 5/5 (100) 2.95 ± 0.17 | 3/3 (100) | |
6 | 0/5 (0) | 5/5 (100) 3.19 ± 0.15 | 3/3 (100) | |
DS-St + EtOH | 1 | 0/5 (0) | 5/5 (100) 1.30 ± 0.28 | 4/4 (100) |
2 | 0/5 (0) | 5/5 (100) 2.20 ± 0.20 | 4/4 (100) | |
3 | 0/5 (0) | 5/5 (100) 3.03 ± 0.63 | 4/4 (100) | |
4 | 0/5 (0) | 5/5 (100) 1.94 ± 0.37 | 4/4 (100) | |
6 | 0/5 (0) | 5/5 (100) 2.77 ± 0.38 | 4/4 (100) | |