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Fig. 3 | Parasites & Vectors

Fig. 3

From: Hepatic myofibroblasts derived from Schistosoma mansoni-infected mice are a source of IL-5 and eotaxin: controls of eosinophil populations in vitro

Fig. 3

Collagen measurement and analysis of GR-MF spheroids a Collagen measurement into supernatants from non-stimulated or TGF-β (30 ng/mL) stimulated cells after 24 h of culture in monolayer b Representative photomicrographs of spheroids stained with haematoxylin/eosin (HE) and Modified Sirius Redstaining for collagen, optical microscope analyses (middle column) and confocal microscope analyses (right column). c Collagen measurement into supernatants from nonstimulated, TGF-β (30 ng/mL) or IL-13 (40 ng/mL) stimulated spheroids after 4 days of culture. Each bar represents the mean ± SEM, n = 3. This graph is representative from three experiments. *P ≤ 0.05 compared with non-stimulated cells

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