Fig. 6

Identification of a second, smaller cell population in SGs. a-f Maximum intensity projection (MIP) images or single slices (unlabeled) of An, stephensi distal lateral lobes exhibiting small nuclei stained with DAPI (A-F), Nile Red (A-D, F), and: mtTFA (a), KDEL (b), GM130 (c), α-tubulin (d), lamin (e), or SG6 and AAPP (f). The small nucleus phenotype is not restricted to the DL lobe or to females (Table 4). Results in (a) show different cell shapes on opposing sides of this DL lobe, cup shaped (dashed box) and squamous (yellow arrows/solid box). The squamous cells contain multiple DAPI bodies of two size classes (large and small). Small and large nuclei are sometimes found in the same cell, but are also observed alone in separate cells (Aiv-vi, two cells: left—small nucleus; right—large nucleus and small nucleus). Small nucleus—containing cells (yellow arrows) typically have a much greater nucleus to cytoplasm ratio than large nucleus-containing secretory cells (white arrows). Small nucleus-containing cells are enriched for mtTFA (a), α-tubulin (d), and AAPP (f). KDEL (b), GM130 (c), and SG6 (f) are present, but not enriched, in small nucleus-containing cells. GM130 and mtTFA levels are high in the transition zone (Ai and Ci, red arrows). Lamin (nuclear envelope) is robust in the M lobe (Eii) and many DL lobes (Eiii), but is frequently disrupted or missing in secretory cells (Eiv-v, white arrows) in lobes where small nucleus-containing cells (Eiv-v, yellow arrows) are seen. g Scale bar lengths are: 50 microns—Ai, Bi, Ci, Ei, Eiii; 20 microns—Aii, Bii, Cii, Ciii, Di, Dii, Eii, Fi; 10 microns—Aiii, Eiv, Fii