Fig. 3

Parasite loads in wild-type, p47phox ^−/−or Nos2 ^−/−mice infected with different strains of L. infantum. The parasite burden in wild-type, p47phox ^−/−or Nos2 ^−/− mice infected with L. infantum ITMAP-263 (a, b), IMT-151 (c, d) or IMT-202 (e, f) strains (liver and spleen, respectively) was determined by serial dilutions of organ homogenates at 15 and 60 days after infection. Each symbol represents one animal and the horizontal bars represent the mean log₁₀ of the number of parasites per organ. Two-way ANOVA with Bonferroni post-hoc test was performed considering the variables “Time” and “Mouse strain”. For clarity, only statistically significant differences between mouse strains are indicated in the graphs: *p < 0.05; **p < 0.01; ****p < 0.0001