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Table 2 Enzyme kinetics of recombinant TgGlo1 proteins: a wild type and mutants produced by point-mutagenesis on active sites of TgGlo1

From: Characterization of Toxoplasma gondii glyoxalase 1 and evaluation of inhibitory effects of curcumin on the enzyme and parasite cultures

  E92Q   R188E    rTgGlo1
  Phase 1 Phase 2 E166Q Phase 1 Phase 2 E251Q E166Q /E251Q Phase 1 Phase 2
Km (μM) 11 ± 2.1 42 ± 5.2 173 ± 8.0 14 ± 2.3 42 ± 2.1 94 ± 3.2 190 ± 5.2 10.0 ± 0.6 38.6 ± 1.5
Kcat (s−1) 159 ± 16 76 ± 9 93 ± 4 654 ± 12 254 ± 20 100 ± 17 114 ± 9 1305 ± 38 257 ± 11
  1. (Phase 1, 1/[S] = less than 40 μM; Phase 2, 1/[S] = more than 40 μM)