Skip to main content

Table 2 Enzyme kinetics of recombinant TgGlo1 proteins: a wild type and mutants produced by point-mutagenesis on active sites of TgGlo1

From: Characterization of Toxoplasma gondii glyoxalase 1 and evaluation of inhibitory effects of curcumin on the enzyme and parasite cultures

 

E92Q

 

R188E

  

rTgGlo1

 

Phase 1

Phase 2

E166Q

Phase 1

Phase 2

E251Q

E166Q /E251Q

Phase 1

Phase 2

Km (μM)

11 ± 2.1

42 ± 5.2

173 ± 8.0

14 ± 2.3

42 ± 2.1

94 ± 3.2

190 ± 5.2

10.0 ± 0.6

38.6 ± 1.5

Kcat (s−1)

159 ± 16

76 ± 9

93 ± 4

654 ± 12

254 ± 20

100 ± 17

114 ± 9

1305 ± 38

257 ± 11

  1. (Phase 1, 1/[S] = less than 40 μM; Phase 2, 1/[S] = more than 40 μM)
Back to article page

Parasites & Vectors

ISSN: 1756-3305

Contact us