Table 1 Primers for PCR amplification of the T. lewisi maxicircle
From: Analysis of the mitochondrial maxicircle of Trypanosoma lewisi, a neglected human pathogen
Primer | Sequence (5’ → 3’) | Fragment positiona |
|---|---|---|
Tl Frag1-F | GCTAATTGCACTAATCGAGGT | (−552)-1446 |
Tl Frag1-R | GCTGGCATCCATTTCTGACT | |
Tl Frag2-F | AAAGGTCCGAGCAGGTTA | 977-3001 |
Tl Frag2-R | CTTTTCTGTGCCACGATGT | |
Tl Frag3-F | ATAAGAATAAGAGGGACAAACC | 2877-4831 |
Tl Frag3-R | CGCATCTGAACTCATAAAATAG | |
Tl Frag4-F | AGGTTTTGTAGTGCGTAGTGTAC | 4446-5267 |
Tl Frag4-R | ATTCCATTCATATTGGATAAGC | |
Tl Frag5-F | TTTATTGTGAACGGTTATGCT | 4851-8008 |
Tl Frag5-R | ACAACTTCGGATTGGACCT | |
Tl Frag6-F | ATGGCTGCGAGATAAACAA | 7697-9586 |
Tl Frag6-R | GGCATTAAAACAAAACAACTT | |
Tl Frag7-F | TATTTGGATCATACGCCTTA | 8980-11526 |
Tl Frag7-R | GGAATGATAAAGCGGGAA | |
Tl Frag8-F | AAAATCCGCTAACTAAACACC | 11104-12847 |
Tl Frag8-R | CCTAAGAAAAGGGAACTTCATAC | |
Tl Frag9-F | TATTTCTAATGGGGCTTGTG | 11971-13782 |
Tl Frag9-R | CACAGAAATCGTAATAGCAATAC | |
Tl Frag10-F | GGAAGTTTACTTTTAGGAAGGC | 13644-14639 |
Tl Frag10-R | GTGGATTCATACACCCATGAC | |
Tl Frag11-F | GGAAGGACCAATCCCAGTT | 14306-16095 |
Tl Frag11-R | TGTACGTTACAATTCGGTGTTT | |
Tl DR-1 Fb | CCATTAAAACCAAATTAGGTG | 16855-(−2912) |
Tl DR-1R | GGAGAGAAGGGAAAATAAGG | |
Tl DR-2 Fb | CCATTAAAACCAAATTAGGTG | 16855-(−3218) |
Tl DR-2R | TCGTATAAAGCGATGTGAAAG |