Fig. 7
LmABCB3 completely rescue the severe growth defect phenotype of yeast lacking ScATM1. a LmABCB3 allows the normal growth of ΔATM1 yeast in minimal medium. ΔATM1/ATM1 (control) and (ΔATM1/ATM1 + LmABCB3 (LmABCB3) cells were plated on minimal (SD) glucose media containing (+FOA) or not (−FOA) 1 mg/ml 5′-fluoroorotic acid (FOA) and incubated at 30 °C for three days. b LmABCB3 allows ΔATM1 yeast to use of non-fermentable carbon sources. ΔATM1, ΔATM1/ATM1 and ΔATM1/LmABCB3 cells were diluted into rich (YP) media containing 2 % (w/v) glucose, 3 % (w/v) galactose, 2 % (v/v) glycerol, 2 % (w/v) lactate, or 2 % (v/v) ethanol to an A600 0.05. After 24 (left) or 48 (right) hours of growth at 30 8C, the A600 was measured. Growth is expressed relative to ΔATM1/ATM1 growth in HC glucose