Table 1 Target sequences, primers and probes used to confirm the detection of rickettsiae by qPCR
Quantitative real-time PCR designation and specificity | qPCR Sytem used | Forward primer | Reverse primer | Probe |
|---|---|---|---|---|
Rickettsia felis | Biotin synthase | ATG-TTC-GGG-CTTCCG-GTA-TG | CCG-ATT-CAG-CAGGTT-CTT-CAA | 6-FAM- GCT-GCG-GCGGTA-TTT-TAG-GAA -TGGG-TAMRA |
Rickettsia aeschlimannii | Scal | AAGCGGCACTTTAGGTAAAGAAA | CATGCTCTGCAAATGAACCA | 6FAM-TGGGGAAATATGCCGTATACGCAAGC -TAMRA |
Rickettsia massiliae | R.mass_9666 | CCAACCTTTTGTTGTTGCAC | TTGGATCAGTGTGACGGACT | 6FAM-CACGTGCTGCTTATACCAGCAAACA -TAMRA |
Rickettsia slovaca | R.slov | GCAACGGTTTTTGGTATCGT | AATCGAATGCACCACCACTT | 6FAM- TCCCGTCCCAGCCATTCGTC -TAMRA |