Table 2 Primers of the 8 microsatellite loci in O. hupensis
Locus | Primer sequence (5′ → 3′) | Repeat motif | Annealing tempreture/(°C) | Allele size from field snails (bp) | NO. of mutilplex PCR | GenBank accession No. |
|---|---|---|---|---|---|---|
T1-10 | Pf: TCACTCGGGTGTAATGCT | (GA)38 | 55 | 173–259 | 1 | GU204080 |
Pr: TTTGTTACTGATGGTGGC | ||||||
T4-25 | Pf: CAATAGTTCGACTCGGAAGA | (CT)35 | 52 | 142–228 | 1 | GU204084 |
Pr: CGAGGTATGGCGTTGCTT | ||||||
T4-22 | Pf: TATCCAAGAAGCCGAAAC | (CA)10 | 50 | 224–256 | 1 | GU204083 |
Pr: GAGGAAAGCGAGGTAAGA | ||||||
D11 | Pf: TTCAGTTGTCTTATTTCGTG | (TG)17 | 55 | 141–192 | 1 | GU204223 |
Pr: TAGATGTTCACTGGTTTGTC | ||||||
T5-11 | Pf: ACGCCAGTCTTGGTGTCA | (GT)14 | 55 | 153–210 | 2 | GU204092 |
Pr: TACTTGGGCAGAAGGGTT | ||||||
T6-17 | Pf: GCTGTCCTTTTACCAACTGC | (AC)8 | 55 | 192–248 | 2 | GU204108 |
Pr: TATCAAAGGATTATGCCGAG | ||||||
A18 | Pf: GCCGATGATACAAGACCC | (CT)18 | 60 | 131–256 | 2 | GU204047 |
Pr: GAGAATCTCCAGGCACGC | ||||||
C22 | Pf: CGGTACATCTGGATAGTGG | (CA)21 | 62 | 185–239 | 2 | GU204145 |
Pr: TGCGAAACAGTTGCAGACAC |