Fig. 2

Molecular Phylogenetic analysis of COI gene from Cx. pipiens taxa. A total of 48 sequences within the 54 mentioned in Table 2 were analyzed. The evolutionary history was inferred by using the Maximum Likelihood method based on the Hasegawa-Kishino-Yano model [46]. The tree with the highest log likelihood (−1539.7457) is shown. Initial tree(s) for the heuristic search were obtained by applying the Neighbor-Joining method to a matrix of pairwise distances estimated using the Maximum Composite Likelihood (MCL) approach. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site, numbers are bootstrap coefficients calculated for a 1000 repeats. All positions containing gaps and missing data were eliminated. There were a total of 1150 positions in the final dataset. Evolutionary analyses were conducted in MEGA6 [44]. Taxa names: pip – Cx. pipiens form pipiens, mol - Cx. pipiens form molestus, quin - Cx. quinquefasciatus, hyb – hybrid, unk – unknown or Cx. pipiens sl