Fig. 2

Neighbor-joining tree showing the relationship of Haemonchus contortus 3-phosphoinositide-dependent protein kinase (Hc-PDK-1) with related kinases. The tree was constructed using the Jones-Tayloe-Thornton model in the program MEGA v.6.0. Bootstrap values (>50 %) are shown above or below the branches (1000 iterations). The PDK-1 s of eight nematodes (Caenorhabditis elegans, Ce-PDK-1; C. remani, Cr-PDK-1; Ascaris sum, As-PDK-1; Brugia malayi, Bm-PDK-1; Loa loa, Ll-PDK-1; Strongyloides stercoralis, Ss-PDK-1; S. ratti, Sr-PDK-1; Trichinella spiralis, Ts-PDK-1) and three organisms other than nematodes (Drosophila melanogaster, DSTPK61; Homo sapiens, Hs-PDK-1; Xenopus laevis, Xl-PDK-1). Caenorhabditis elegans Ce-AKT-1 was used as an outgroup. GeneBank accession numbers are listed to the right of individual species names