Citrate (mM) | Activity [U (mg protein) -1] |
---|
| ATP (1 mM) | ATP (5 mM) |
---|
0 | 4.02 ± 0.26 | 2.50 ± 0.13 |
0.25-8.0 | 4.01 ± 0.40 | 2.44 ± 0.19 |
- PFK activity was measured at pH = 7.4, 1 mM F6P at low and high ATP concentrations, without (first row) and with added citrate. The assay was conducted at eight different citrate concentrations in the range of 0.25 – 8 mM. Values are means ± SEM of four independent experiments