Investigating associations between biting time in the malaria vector Anopheles arabiensis Patton and single nucleotide polymorphisms in circadian clock genes: support for sub-structure among An. arabiensis in the Kilombero valley of Tanzania

Table 2 Loci identity showing reference genes, chromosomes of origin, mutated nucleotide and variant codons

SNP ID	Reference gene ID (An. gambiae)	Chromosome	SNP type	Reference (An. gambiae) codon	Variant codon	Reference amino acid	Variant amino acid	Mutation type
PER-PAS-202	AGAP001856	3R	T/C	GAC	GAU	D	D	S
PER-PAS-082	AGAP001856	3R	C/T	GAU	GAC	D	D	S
PER-PAS-301	AGAP001856	3R	G/A	CGG	CGA	R	R	S
PER-PAS-355	AGAP001856	3R	T/C	GGC	GGU	G	G	S
PER-PAS-370	AGAP001856	3R	G/C	CUC	CUG	L	L	S
CRY1_E04-240	AGAP001958	2R	T/C	CGG	UGG	R	W	NS
CRY1_E04-206	AGAP001958	2R	T/A	UCA	UCU	S	S	S
CRY1_E04-097	AGAP001958	2R	A/G	GAA	AAA	E	K	NS
CRY1_E04-252	AGAP001958	2R	T/C	UUU	UUC	F	F	S
CRY2_E05-351	AGAP004261	2R	C/T	CUU	CUC	L	L	S
CRY2_E05-378	AGAP004261	2R	C/T	CUC	CUU	L	L	S
CRY2_E05-407	AGAP004261	2R	G/C	AGG	AGC	R	S	NS
CRY2_E05-501	AGAP004261	2R	A/G	GCA	ACA	A	T	NS
CRY2_E05-561	AGAP004261	2R	T/C	AAC	AAU	N	N	S
CRY2_E05-045	AGAP004261	2R	T/C	GUU	GUC	V	V	S
CRY2_E05-125	AGAP004261	2R	G/A	GCG	ACG	A	T	NS
CYC_E01-268	AGAP005655	2L	T/C	UGC	UGU	C	C	S
CYC_E01-250	AGAP005655	2L	G/T	UCG	GCG	S	A	NS
CYC_E01-217	AGAP005655	2L	C/A	ACC	CCC	T	P	NS
CYC_E01-093	AGAP005655	2L	G/A	ACG	GCG	T	A	NS
CYC_E01-072	AGAP005655	2L	G/C	CUC	CUG	L	L	S
CYC_E01-021	AGAP005655	2L	T/C	AGU	AGC	S	S	S
CYC_E01-034	AGAP005655	2L	T/C	UUC	UUU	F	F	S
Clk_E01-087	AGAP005711	2L	G/A	GAU	AAU	D	N	NS
Clk_E01-192	AGAP005711	2L	C/T	CUC	UUC	L	F	NS
Clk_E01-240	AGAP005711	2L	G/A	CUG	CUA	L	L	S
Clk_E01-267	AGAP005711	2L	C/T	CAC	UAC	H	Y	NS
PDP1_E02-110	AGAP006376	2L	G/A	GCG	GCA	A	A	S
Vri_E02-355	AGAP007801	3R	C/T	AUC	AUU	I	I	S
Vri_E02-427	AGAP007801	3R	C/T	ACC	ACU	T	T	S
TIM_E05-087	AGAP007801	3R	C/A	CCC	CCA	P	P	S
TIM_E05-189	AGAP007801	3R	A/G	AUG	GUG	M	V	NS
TIM_E05-075	AGAP001856	3R	G/C	GAC	CAC	D	H	NS
TIM_05-195	AGAP007801	3R	C/T	GUC	GUU	V	V	NS

The loci were selected from the 8 clock genes; PER period, CRY1 cryptochrome1, CRY2 cryptochrome2, CYC cycle, CLK clock, Pdp1 par domain protein 1, VRI vrille and TIM timeless. Mutation type S means synonymous mutation and NS means non-synonymous mutation

ISSN: 1756-3305