Fig. 4

MDM infection with eGFP labelled L. donovani was analyzed using the BD FACS Calibur. a A representative forward scatter (FSC) and sideward scatter (SSC) plot shows gating for cultured macrophages. b A representative infection assay result for eGFP L. donovani in bovine MDM at two time points of infection. GFP fluorescence was evaluated among gated macrophages from either cross-breed MDM (i) or Zebu cattle (ii). Grey colored plots represent MDM infected with wild type parasite infections, whereas green colored plots depict MDM infected with eGFP-labelled L. donovani. Cells were evaluated after 24 h of infection (upper panels) or 48 h of infection (lower panels)