Table 1 Primers and probes used in tick species determination and pathogen screening
From: Tick-borne bacterial pathogens in southwestern Finland
| Primer/probe name | Primer/probe target | 5’ → 3’ | Reference |
|---|---|---|---|
| qPCR: | |||
| IXO-I2-F4 | Ixodes spp. ITS2 | TCTCGTGGCGTTGATTTGC | This paper |
| IXO-I2-R4 | Ixodes spp. ITS2 | CTGACGGAAGGCTACGACG | |
| Ipe-I2-P4 | I. persulcatus ITS2 | [FAM]-TGCGTGGAAAGAAAACGAG-[BHQ1] | |
| Iri-I2-P4 | I. ricinus ITS2 | [VIC]-TGCTCGAAGGAGAGAACGA-[BHQ1] | |
| Bb23Sf | B. burgdorferi 23S RNA | CGAGTCTTAAAAGGGCGATTTAGT | Courtney et al. [83] |
| Bb23Sr | B. burgdorferi 23S RNA | GCTTCAGCCTGGCCATAAATAG | |
| Bb23Sp | B. burgdorferi 23S RNA | [FAM]-AGATGTGGTAGACCCGAAGCCGAGTG-[BHQ1] | |
| Bmi-F | B. miyamotoi glpQ | CACGACCCAGAAATTGACACA | Vayssier-Taussat et al. [84] |
| Bmi-R | B. miyamotoi glpQ | GTGTGAAGTCAGTGGCGTAAT | |
| Bmi-P | B. miyamotoi glpQ | [FAM]-TCGTCCGTTTTCTCTAGCTCGATTGGG-[BHQ1] | |
| Bart-ssRA-F | Bartonella ssRa | GCTATGGTAATAAATGGACAATGAAATAA | Diaz et al. [85] |
| Bart-ssRA-R | Bartonella ssRa | GCTTCTGTTGCCAGGTG | |
| Bart-ssRA-P | Bartonella ssRa | [FAM]-ACCCCGCTTAAACCTGCGACG-[BHQ1] | |
| Rspp-F | Rickettsia gltA | GAGAGAAAATTATATCCAAATGTTGAT | Labruna et al. [86] |
| Rspp-R | Rickettsia gltA | AGGGTCTTCGTGCATTTCTT | |
| Rspp-P | Rickettsia gltA | [CY5]-CATTGTGCCATCCAGCCTACGGT-[BHQ3] | |
| CNe-F | Ca. N. mikurensis groEL | AGAGACATCATTCGCATTTTGGA | Vayssier-Taussat et al. [84] |
| CNe-R | Ca. N. mikurensis groEL | TTCCGGTGTACCATAAGGCTT | |
| CNe-P | Ca. N. mikurensis groEL | [TAMRA]-AGATGCTGTTGGATGTACTGCTGGACC-[BHQ2] | |
| PCR: | |||
| CS877f | Rickettsia gltA | TAATACGACTCACTATAGGGGGGGACCTGCTCACGGCGG | Mediannikov et al. [87] |
| CS1258r | Rickettsia gltA | ATTAACCCTCACTAAAGATTGCAAAAAGTACAGTGAACA |