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Table 2 Molecular prevalence of L. infantum, filariae and Wolbachia in dogs with clinical signs compatible with leishmaniosis and/or dirofilariosis (n = 75)

From: Molecular detection of Leishmania infantum, filariae and Wolbachia spp. in dogs from southern Portugal

Variable/category

No. (%) of tested dogs

Percentage (n) of positive dogs

PCR

PCR

PCR

≥1 agent

L. infantum

filariae

Wolbachia spp.

Gender

75

    

 Female

32 (42.7)

28.1 (9)a

12.5 (4)

9.4 (3)

34.4 (11)

 Male

43 (57.3)

53.5 (23)a

7.0 (3)

7.0 (3)

55.8 (24)

Age (months)

67

    

 [1–11]

1 (1.5)

0.0 (0)

0.0 (0)

0.0 (0)

0.0 (0)

 [12–83]

33 (49.3)

39.4 (13)

12.1 (4)

12.1 (4)

45.5 (15)

 [84–192]

33 (49.3)

42.4 (14)

9.1 (3)

6.1 (2)

45.5 (15)

Breed

73

    

 Defined (incl. crosses)

40 (54.8)

40.0 (16)

7.5 (3)

7.5 (3)

42.5 (17)

 Mongrel

33 (45.2)

48.5 (16)

12.1 (4)

9.1 (3)

54.5 (18)

Aptitude

75

    

 Pet

37 (49.3)

40.5 (15)

16.2 (6)

13.5 (5)

48.6 (18)

 Other1

38 (50.7)

44.7 (17)

2.6 (1)

2.6 (1)

44.7 (17)

Housing

41

    

 Indoors and mixed

18 (43.9)

27.8 (5)

5.6 (1)

5.6 (1)

33.3 (6)

 Outdoors

23 (56.1)

47.8 (11)

17.4 (4)

13.0 (3)

52.2 (12)

Repellent insecticides2

55

    

 Yes

19 (34.5)

21.1 (4)b

10.5 (2)

5.3 (1)

26.3 (5)

 No

36 (65.5)

52.8 (19)b

5.6 (2)

5.6 (2)

55.6 (20)

Macrocyclic lactones3

37

    

 Yes

9 (24.3)

0.0 (0)

0.0 (0)

0.0 (0)

0.0 (0)

 No

28 (75.7)

25.0 (7)

10.7 (3)

7.1 (2)

32.1 (9)

Total

75 (100)

42.7 (32)

9.3 (7*)

8.0 (6)

46.7 (35)

  1. 1Farm, guard, hunting or stray; 2Deltamethrin or permethrin; 3Ivermectin, milbemycin oxime or moxydectin; a P = 0.050; b P = 0.048; *Comprising six dogs positive for D. immitis and another one for A. reconditum