Fig. 3

Expression and localization of PbPH in the parasites. a Western blot of PbPH in asexual- and sexual-stage parasites. Parasite antigens of schizonts, gametocytes, and ookinetes (10 μg/lane) were incubated with anti-PbPH sera (1:200) from immunized mice. Protein loading is estimated using anti-Hsp70 serum (1:200). b IFA analysis. Blood smears containing parasite samples were stained at different times after collection of P. berghei-infected blood. Parasites were fixed and stained with anti-PbPH sera (1:200) from recombinant PbPH-immunized mice and then with FITC-conjugated goat anti-mouse IgG (green). Nuclei were stained by DAPI (blue). Pbs21 mAb clone 13.1 serves as a positive control. Scale - bar: 5 μm