Fig. 4

Knockout of pbph gene from P. berghei parasite. a A schematic shows the wild type pbph locus, transfection construct, and the recombined locus. Through a double-crossover strategy the pbph locus was replaced with the dhfr-expressing cassette. Primers 1–6 used to detect gene knockout are marked. b PCR detection of wild-type and Δpbph parasite genomes. Lane 1: primers 1 + 2 (910 bp); Lane 2: primer 1 + 3 (990 bp); and Lane 3: primer 5 + 6 (1, 040 bp)