Fig. 2
a Midgut DENV titre in Ae. aegypti fed directly on patients (blue circles) and those fed patient’s EDTA blood via membrane feeding assay (MFA) (red squares). Midgut DENV titre were expressed as Log10 copies/midgut. *denotes significant difference, P < 0.05. Each point represents an individual midgut. Note: Due to small sample size, Mann–Whitney U-tests were not conducted for underlined patients’ numbers (1, 2 and 10). b DENV salivary gland titre in Ae. aegypti fed directly on patients (blue circles) and those fed patient’s EDTA blood (red squares). DENV titres were expressed as Log10 TCID50/ml. *denotes significant difference, P < 0.05. Each point represents an individual salivary gland. Note: Due to absence of disseminated infection (patient no 1, 2, 7, 9, 10, 14 and 17) or small sample size (4, 15, 18, 19 and 24), Mann–Whitney U-tests were not conducted for these feeding events (underlined patients’ numbers)