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Table 3 Absolute amounts of sterols of stationary and metacyclic promastigotes of a virulent strain of Leishmania infantum, incubated in the absence or presence of MβCDa

From: Dynamics of sterol synthesis during development of Leishmania spp. parasites to their virulent form

Sterol Stationary (n = 6) Metacyclic (n = 6) MβCD-metacyclic (n = 6)
Cholesterol 448.3 (249.2) 248.9 (120.8) 89.0 (40.6)*
Ergosterol -I 331.0 (290.5) 68.4 (13.1) 40.3 (22.3)*
Zymosterol 44.2 (108.2) 0.0 (0.0) 0.0 (0.0)
Ergosta-7,22-dien-3β-ol - I 81.8 (40.6) 100.6 (45.8) 49.3 (17.2)*
Ergostatetraenol 0.0 (0.0) 0.0 (0.0) 0.0 (0.0)
Ergosterol - II 403.6 (437.7) 35.7 (33.4) 11.5 (9.3)
Ergosta-7,22-dien-3β-ol - II 387.8 (282.1) 318.9 (137.8) 81.8 (37.3)**
Lanosterol 6.4 (15.7) 0.0 (0.0) 0.0 (0.0)
Stigmasta-7,24(28)-dien-3β-ol 62.8 (63.6) 37.8 (19.4) 17.5 (8.6)*
Total 1766.0 (1307.8) 810.2 (239.2) 289.5 (120.7)**
  1. aNumbers indicate the mean (SD) absolute concentrations of sterols (ng/107 cells). Metacyclic promastigotes were isolated from stationary phase promastigotes as previously described [3]. An aliquot of the metacyclic cells were treated with 25 mM MβCD
  2. *P < 0.05; **P < 0.01 between metacyclic and MβCD-treated metacyclic promastigotes