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Table 2 Details of primers, PCR type performed and band size (bp) obtained for An. culicifacies sibling species in the present study

From: Malaria vector Anopheles culicifacies sibling species differentiation using egg morphometry and morphology

Sequence (5′-3′) Primer AS-PCR Type Band size for different sibling species of An. culicifacies
GAC CCG TCT TGA AAC ACG GA D3A D3 PCR differentiates sibling species in two groups one is A & D group and second is B, C & E group 382 & 313 bp for species A & D
TCG GAA GGA ACC AGC TAC TA D3B 385 & 133 bp for species B, C & E
GCC GTC CCC ATA CAC TG ACA
CCG TAA TCC CGT GAT AAC TT ACB
CTA ATC GAT ATT TAT TAC AC ADF A/D-PCR differentiates sibling species A & D individually 359 bp for species A
TTA CTC CTA AAG AAG GC ADR 248 bp for species B
TTA GAG TTT GAT TCT TAC DF 248 & 95 bp for species C
AAA TTA TTT GAA CAG TAT TG BCEF B/C/E- PCR differentiates sibling species B, C & E individually 359 & 166 bp for species D
TTA TTT ATT GGT AAA ACA AC BCR 248 & 178 bp for species E
AGG AGT ATT AAT TTC GTC T CR
GTA AGA ATC AAA TTC TAA G ER