Table 2 Details of primers, PCR type performed and band size (bp) obtained for An. culicifacies sibling species in the present study
Sequence (5′-3′) | Primer | AS-PCR Type | Band size for different sibling species of An. culicifacies |
|---|---|---|---|
GAC CCG TCT TGA AAC ACG GA | D3A | D3 PCR differentiates sibling species in two groups one is A & D group and second is B, C & E group | 382 & 313 bp for species A & D |
TCG GAA GGA ACC AGC TAC TA | D3B | 385 & 133 bp for species B, C & E | |
GCC GTC CCC ATA CAC TG | ACA | ||
CCG TAA TCC CGT GAT AAC TT | ACB | ||
CTA ATC GAT ATT TAT TAC AC | ADF | A/D-PCR differentiates sibling species A & D individually | 359Â bp for species A |
TTA CTC CTA AAG AAG GC | ADR | 248Â bp for species B | |
TTA GAG TTT GAT TCT TAC | DF | 248 & 95Â bp for species C | |
AAA TTA TTT GAA CAG TAT TG | BCEF | B/C/E- PCR differentiates sibling species B, C & E individually | 359 & 166 bp for species D |
TTA TTT ATT GGT AAA ACA AC | BCR | 248 & 178Â bp for species E | |
AGG AGT ATT AAT TTC GTC T | CR | ||
GTA AGA ATC AAA TTC TAA G | ER |