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Table 3 Morphometric attributes of Anopheles culicifacies sibling species A, D and E eggs

From: Malaria vector Anopheles culicifacies sibling species differentiation using egg morphometry and morphology

Abbreviations An. culicifacies A Mean ± SE (Range) An. culicifacies D Mean ± SE (Range) An. culicifacies E Mean ± SE (Range) F-value P-value
Egg attributes
 EGGL 415.75 ± 3.63a (406.00–432.00) 391.24 ± 8.94 (366.30–411.70) 409.03 ± 2.79 (404.80–414.30) 4.532 0.037
 EGGW 124.42 ± 2.82a (114.00–134.00) 137.76 ± 3.40 (128.70–145.18) 140.13 ± 6.55 (130.00–152.40) 5.315 0.024
 ELWR 3.34 ± 0.06 (3.10–3.56) 2.84 ± 0.07 (2.64–3.10) 2.92 ± 0.12 (2.72–3.14) 13.072 0.001
Deck attributes
 DECL 400 ± 6.06a (380.00–416.00) 340.80 ± 15.95 (316.00–403.92) 395.50 ± 4.37 (390.30–404.20) 8.599 0.007
 DECW 41.20 ± 1.96a (36.00–48.00) 44.12 ± 4.74 (40.20–54.00) 15.60 ± 0.74 (14.16–16.64) 21.166 < 0.001
 DEMW 33.60 ± 2.78a (28.00–44.00) 34.96 ± 2.79 (29.84–40.00) 9.93 ± 1.00 (8.00–11.40) 23.440 < 0.001
 DECA 14,968 ± 1016.04a (12,800.00–18,768.00) 12,824.75 ± 115.48 (10,287.00–14,852.00) 6174.90 ± 349.48 (5526.90–6725.80) 18.409 < 0.001
Ventral tubercles
 ATUN 3.75 ± 0.25 (3.00–5.00) 2.60 ± 0.24 (2.00–3.00) 3.20 ± 0.20 (3.00–4.00) 5.606 0.015
 PTUN 3.12 ± 0.12 (3.00–4.00) 2.60 ± 0.24 (2.00–3.00) 2.60 ± 0.40 (2.00–4.00) 1.742 0.209
 ATLN 5.62 ± 0.15 (5.00–6.33) 7.24 ± 0.64 (6.00–9.00) 7.10 ± 0.29 (6.66–7.66) 8.036 0.006
 PTLN 5.85 ± 0.35 (5.85–8.00) 7.23 ± 0.64 (6.00–9.00) 7.00 ± 0.28 (6.50–7.50) 2.920 0.093
Micropyle
 MICA 326.32 ± 10.41a (291.00–380.00) 252.52 ± 7.21 (236.60–271.60) 226.60 ± 20.05 (188.60–256.70) 19.114 < 0.001
 MICDR 50.88 ± 1.10 (46.98–54.35) 42.05 ± 1.19 (39.42–45.26) 37.76 ± 3.34 (31.43–42.78) 17.453 < 0.001
 MIDN 6.42 ± 0.20 (6.00–7.00) 6.00 + 0.00 (6.00–6.00) 6.00 + 0.00 (6.00–6.00) 2.062 0.174
Float attributes
 FLOL 283.07 ± 4.27a (264.00–297.00) 250.06 ± 6.73 (232.00–268.94) 293.57 ± 4.22 (285.60–300.00) 15.445 < 0.001
 FLOW 73.70 ± 2.04a (70.00–82.92) 66.12 ± 2.48 (59.40–71.40) 89.76 ± 2.74 (84.90–94.40) 18.266 < 0.001
 FLWR 3.86 ± 0.11 (3.30–4.22) 3.85 ± 0.11 (3.66–4.06) 3.27 ± 0.13 (3.02–3.47) 5.718 0.020
 FRIN 24.0 ± 0.95 (22.00–27.00) 18.60 ± 0.40 (18.00–20.00) 22.00 ± 0.57 (21.00–23.00) 11.833 0.001
 FLELP 68.0 ± 1.32 (61.11–71.42) 63.89 ± 0.88 (61.55–65.90) 71.76 ± 1.44 (68.90–73.50) 7.170 0.009
 FLEWR 2.25 ± 0.05 (2.09–2.49) 1.82 ± 0.03 (1.74–1.91) 2.10 ± 0.12 (1.87–2.30) 14.710 < 0.001
 FWRR 3.09 ± 0.12 (2.66–3.63) 3.62 ± 0.10 (3.30–3.75) 4.08 ± 0.19 (3.69–4.29) 3.436 0.066
 FLPR 11.94 ± 0.41 (10.52–13.45) 13.44 ± 0.53 (11.60–14.94) 13.36 ± 0.46 (12.83–14.28) 3.436 0.066
 FWEWR 0.58 ± 0.01 (0.53–0.63) 0.47 ± 0.01 (0.43–0.52) 0.64 ± 0.02 (0.61–0.70) 13.898 < 0.001
  1. EGGL length of egg, EGWL width of egg, ELWR length/width ratio of egg, DECL maximum length of deck, DECW maximum width of anterior deck, DEMW maximum width of middle deck, DECA area of deck, MICA area of micropylar disc, MICDR area/sector number ratio of micropylar disc, MIDN number of sector of micropylar disc, ATUN number of antero-ventral tubercles, PTUN number of postero-ventral tubercles, ATLN number of lobes of antero-ventral tubercles, PTLN number of lobes of postero-ventral tubercles FLOL maximum length of float, FLOW maximum width of float, FLWR length/width ratio of egg, FRIN number of float ribs, FLELP float length as % of egg length, FLEWR float length / egg width ratio, FWRR float width / number of ribs ratio, FLRR float length / number of ribs, FWEWR float width / egg width ratio. a = μm, a = μm2