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Table 3 Morphometric attributes of Anopheles culicifacies sibling species A, D and E eggs

From: Malaria vector Anopheles culicifacies sibling species differentiation using egg morphometry and morphology

Abbreviations

An. culicifacies A Mean ± SE (Range)

An. culicifacies D Mean ± SE (Range)

An. culicifacies E Mean ± SE (Range)

F-value

P-value

Egg attributes

 EGGL

415.75 ± 3.63a (406.00–432.00)

391.24 ± 8.94 (366.30–411.70)

409.03 ± 2.79 (404.80–414.30)

4.532

0.037

 EGGW

124.42 ± 2.82a (114.00–134.00)

137.76 ± 3.40 (128.70–145.18)

140.13 ± 6.55 (130.00–152.40)

5.315

0.024

 ELWR

3.34 ± 0.06 (3.10–3.56)

2.84 ± 0.07 (2.64–3.10)

2.92 ± 0.12 (2.72–3.14)

13.072

0.001

Deck attributes

 DECL

400 ± 6.06a (380.00–416.00)

340.80 ± 15.95 (316.00–403.92)

395.50 ± 4.37 (390.30–404.20)

8.599

0.007

 DECW

41.20 ± 1.96a (36.00–48.00)

44.12 ± 4.74 (40.20–54.00)

15.60 ± 0.74 (14.16–16.64)

21.166

< 0.001

 DEMW

33.60 ± 2.78a (28.00–44.00)

34.96 ± 2.79 (29.84–40.00)

9.93 ± 1.00 (8.00–11.40)

23.440

< 0.001

 DECA

14,968 ± 1016.04a (12,800.00–18,768.00)

12,824.75 ± 115.48 (10,287.00–14,852.00)

6174.90 ± 349.48 (5526.90–6725.80)

18.409

< 0.001

Ventral tubercles

 ATUN

3.75 ± 0.25 (3.00–5.00)

2.60 ± 0.24 (2.00–3.00)

3.20 ± 0.20 (3.00–4.00)

5.606

0.015

 PTUN

3.12 ± 0.12 (3.00–4.00)

2.60 ± 0.24 (2.00–3.00)

2.60 ± 0.40 (2.00–4.00)

1.742

0.209

 ATLN

5.62 ± 0.15 (5.00–6.33)

7.24 ± 0.64 (6.00–9.00)

7.10 ± 0.29 (6.66–7.66)

8.036

0.006

 PTLN

5.85 ± 0.35 (5.85–8.00)

7.23 ± 0.64 (6.00–9.00)

7.00 ± 0.28 (6.50–7.50)

2.920

0.093

Micropyle

 MICA

326.32 ± 10.41a (291.00–380.00)

252.52 ± 7.21 (236.60–271.60)

226.60 ± 20.05 (188.60–256.70)

19.114

< 0.001

 MICDR

50.88 ± 1.10 (46.98–54.35)

42.05 ± 1.19 (39.42–45.26)

37.76 ± 3.34 (31.43–42.78)

17.453

< 0.001

 MIDN

6.42 ± 0.20 (6.00–7.00)

6.00 + 0.00 (6.00–6.00)

6.00 + 0.00 (6.00–6.00)

2.062

0.174

Float attributes

 FLOL

283.07 ± 4.27a (264.00–297.00)

250.06 ± 6.73 (232.00–268.94)

293.57 ± 4.22 (285.60–300.00)

15.445

< 0.001

 FLOW

73.70 ± 2.04a (70.00–82.92)

66.12 ± 2.48 (59.40–71.40)

89.76 ± 2.74 (84.90–94.40)

18.266

< 0.001

 FLWR

3.86 ± 0.11 (3.30–4.22)

3.85 ± 0.11 (3.66–4.06)

3.27 ± 0.13 (3.02–3.47)

5.718

0.020

 FRIN

24.0 ± 0.95 (22.00–27.00)

18.60 ± 0.40 (18.00–20.00)

22.00 ± 0.57 (21.00–23.00)

11.833

0.001

 FLELP

68.0 ± 1.32 (61.11–71.42)

63.89 ± 0.88 (61.55–65.90)

71.76 ± 1.44 (68.90–73.50)

7.170

0.009

 FLEWR

2.25 ± 0.05 (2.09–2.49)

1.82 ± 0.03 (1.74–1.91)

2.10 ± 0.12 (1.87–2.30)

14.710

< 0.001

 FWRR

3.09 ± 0.12 (2.66–3.63)

3.62 ± 0.10 (3.30–3.75)

4.08 ± 0.19 (3.69–4.29)

3.436

0.066

 FLPR

11.94 ± 0.41 (10.52–13.45)

13.44 ± 0.53 (11.60–14.94)

13.36 ± 0.46 (12.83–14.28)

3.436

0.066

 FWEWR

0.58 ± 0.01 (0.53–0.63)

0.47 ± 0.01 (0.43–0.52)

0.64 ± 0.02 (0.61–0.70)

13.898

< 0.001

  1. EGGL length of egg, EGWL width of egg, ELWR length/width ratio of egg, DECL maximum length of deck, DECW maximum width of anterior deck, DEMW maximum width of middle deck, DECA area of deck, MICA area of micropylar disc, MICDR area/sector number ratio of micropylar disc, MIDN number of sector of micropylar disc, ATUN number of antero-ventral tubercles, PTUN number of postero-ventral tubercles, ATLN number of lobes of antero-ventral tubercles, PTLN number of lobes of postero-ventral tubercles FLOL maximum length of float, FLOW maximum width of float, FLWR length/width ratio of egg, FRIN number of float ribs, FLELP float length as % of egg length, FLEWR float length / egg width ratio, FWRR float width / number of ribs ratio, FLRR float length / number of ribs, FWEWR float width / egg width ratio. a = μm, a = μm2