Table 1 Primers used in the present study for evaluation of expression of the ABC subfamily B genes (ABCB1, ABCB6, ABCB8 and ABCB10) in the Ixodes ricinus cell line IRE/CTVM19
Primer sequences | ||||
|---|---|---|---|---|
ABCB1 | F: | 5′ – | TCTTTGCCGTCTTCTACAG | – 3′ |
R: | 5′ – | CAGGTTCTCTCCAGCGAT | – 3′ | |
ABCB6 | F: | 5′ – | AGACTATGTCCTCTTCCTCA | – 3′ |
R: | 5′ – | CATCTATCACCTCTGCCTT | – 3′ | |
ABCB8 | F: | 5′ – | ATCAGGAACGCCGACATC | – 3′ |
R: | 5′ – | AGTTTCCAGTAGACACCCTT | – 3′ | |
ABCB10 | F: | 5′ – | TGTCCTAACCATTGCTCACA | – 3′ |
R: | 5′ – | TGATGTTCCACTAATGTCCG | – 3′ | |
β-Actin | F: | 5′ – | CACGGCATCGTGACCAACTG | – 3′ |
R: | 5′ – | CGAACATGATCTGAGTCATCTTCTC | – 3′ | |