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Fig. 1 | Parasites & Vectors

Fig. 1

From: Protection against Schistosoma mansoni infection using a Fasciola hepatica-derived fatty acid binding protein from different delivery systems

Fig. 1

The expression and purification of rFh15b using the baculovirus system. a The generated vector pFBFh15His; b The nucleotide sequence from Fh15, including the Kozak sequence, the C-terminus 6-His tag and the restriction sequences for BamHI and Xbal; c The expression of rFh15b detected with Coomassie blue staining (Lane 1, molecular weight marker; Lane 2: non-induced baculovirus; Lane 3, induced baculovirus) and Western blot using anti-6His monoclonal antibody (Lane 1, molecular weight marker; Lane 2, induced baculovirus; Lane 3: non-induced baculovirus); d Purification of rFh15b by affinity chromatography detected by Coomassie blue staining and Western blot using anti-6His monoclonal antibody

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Parasites & Vectors

ISSN: 1756-3305

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