From: Molecular detection of tick-borne bacteria and protozoa in cervids and wild boars from Portugal
Pathogen | Target gene | Oligonucleotide sequences (5′-3′) | Amplicon size (bp) | Reference | ||
---|---|---|---|---|---|---|
Forward | Reverse | |||||
Anaplasma spp./Ehrlichia spp. | 16S rRNA | GGTACCYACAGAAGAAGTCC | TAGCACTCATCGTTTACAGC | 345 | [38] | |
groEL | ACTGATGGTATGCARTTTGAYCG | TCTTTRCGTTCYTTMACYTCAACTTC | 600 | [39] | ||
Anaplasma marginale/A. centrale/A. ovis | msp4 | GGGAGCTCCTATGAATTACAGAGAATTGTTTAC | CCGGATCCTTAGCTGAACAGGAATCTTGC | 851 | [40] | |
Anaplasma phagocytophilum | msp4 | ATGAATTACAGAGAATTGCTTGTAGG | TTAATTGAAAGCAAATCTTGCTCCTATG | 849 | [13] | |
Babesia spp./Theileria spp. | 18S rRNA | AATACCCAATCCTGACACAGGG | TTAAATACGAATGCCCCCAAC | 400 | [38] | |
Borrelia burgdorferi (sensu lato) | ITS 5S-23S rRNA | Outer primmers | ACCATAGACTCTTATTACTTTGAC | TAAGCTGACTAATACTAATTACCC | 380 | [41] |
Inner primers | ACCATAGACTCTTATTACTTTGACCA | GAGAGTAGGTTATTGCCAGGG | 225 | |||
flaB | Outer primmers | TGGTATGGGAGTTTCTGG | TAAGCTGACTAATACTAATTACCC | 774 | [42] | |
Inner primers | CAGACAACAGAGGGAAAT | TCAAGTCTATTTTGGAAAGCACC | 604 | |||
Rickettsia spp. | gtlA | GGGGGCCTGCTCACGGCGG | ATTGCAAAAAGTACAGTGAACA | 381 | [43] |