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Table 2 Cross-reactivity investigation for LD RPA assay

From: Mobile suitcase laboratory for rapid detection of Leishmania donovani using recombinase polymerase amplification assay

Pathogen name Number of isolates Sample type and reference test LD RPA assay results Source
L. donovani MHOM/ET/67/HU3Z18 1 Reference DNA with concentration of around 40–100 ng/μl Positive Paul-Ehrlich-Institute, Langen, Germany
L. major MHOM/IL/81/FEBNI 1 Positive
L. aethiopica MHOM/ET/72/L100Z14 1 Positive
L. infantum (50134D) MHOM/TN/80/IPT-1 1 Positive American Type Culture Collection, Manassas, USA
L. tropica (50129D) 1 Negative
L. amazonensis 1 Negative Dept. of Microbiology, University Medical Center Gottingen, Germany
L. braziliensis 1 Negative
Toxoplasma gondii 1 Negative
Plasmodium falciparum 3 DNA extracted from blood samples of malaria patients. Plasmodium species-specific nested PCR was performed [40]. Negative Icddr, b
Plasmodium vivax 3 Negative
Salmonella typhi 1 DNA extracted from culture Negative
Mycobacterium tuberculosis 2 DNA extracted from lymph node aspirate from two extra pulmonary TB patients. Both were positive in culture and IS6110 PCR [41]. Negative