Table 1 Transcriptional level of selected genes of Rickettsia rickettsii in salivary glands of ticks exposed to temperature upshift, blood-feeding or both stimuli simultaneously. Microfluidic RT-qPCR fold changes (mean of at least nine biological replicates) with statistically significant differences with respect to control are presented
Temperature upshift | Blood-feeding | Temperature upshift + Blood-feeding | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Female | Male | Female | Male | Female | Male | ||||||||
Gene ID | Annotation | Fold change | W a (P-value) | Fold change | W (P-value) | Fold change | W (P-value) | Fold change | W (P-value) | Fold change | W (P-value) | Fold change | W (P-value) |
A1G_00075 | Ankyrin repeat-containing protein | nsb | ns | ns | ns | 2.95 | 77 (0.0160) | 2.39 | 74 (0.0415) | ||||
A1G_01255 | Ankyrin repeat-containing protein | ns | ns | -1.92 | 5 (0.0012) | ns | ns | ns | |||||
A1G_00615 | Preprotein translocase subunit SecB | ns | ns | -4.17 | 0 (0.0001) | -1.96 | 12 (0.0221) | -2.63 | 10 (0.0122) | -2.17 | 14 (0.0277) | ||
A1G_01005 | Preprotein translocase subunit SecE | 1.54 | 94 (0.0050) | 1.79 | 100 (0.0009) | ns | ns | 1.55 | 90 (0.0002) | 1.73 | 90 (0.0002) | ||
A1G_04855 | Preprotein translocase subunit SecA | ns | -1.54 | 13 (0.0290) | ns | ns | ns | -1.56 | 4 (0.0013) | ||||
A1G_05455 | Preprotein translocase subunit SecY | 2.94 | 94 (0.0050) | ns | -1.64 | 5 (0.0012) | ns | 1.79 | 75 (0.0242) | ns | |||
A1G_02210 | Type IV secretion system component virB8 protein | ns | ns | ns | ns | 2.44 | 86 (0.0011) | 1.96 | 86 (0.0013) | ||||
A1G_06670 | Type IV secretion system component virB4 protein precursor | 1.69 | 88 (0.0182) | ns | ns | ns | 2.06 | 84 (0.0021) | 1.59 | 88 (0.0005) | |||
A1G_07055 | Ferredoxin | ns | ns | ns | ns | 1.61 | 82 (0.0040) | ns | |||||
A1G_02555 | Thioredoxin peroxidase 1 | ns | 1.57 | 90 (0.0154) | 1.74 | 90 (0.0001) | ns | 2.32 | 90 (0.0002) | 2.04 | 90 (0.0002) | ||
A1G_00010 | Thioredoxin | ns | 1.71 | 97 (0.0021) | ns | ns | 1.99 | 89 (0.0003) | 2.01 | 90 (0.0002) | |||
A1G_01335 | Molecular chaperone DnaK | -1.72 | 11 (0.0143) | ns | -2.22 | 1 (0.0002) | -1.52 | 10 (0.0144) | -3.85 | 0 (0.0002) | -2.44 | 4 (0.0013) | |
A1G_01505 | Co-chaperone HscB | ns | 1.84 | 99 (0.0009) | ns | ns | ns | 1.93 | 89 (0.0003) | ||||
A1G_01545 | DNA gyrase subunit A | ns | ns | -2.70 | 2 (0.0004) | -2.22 | 14 (0.0332) | -2.08 | 16 (0.0300) | ns | |||
A1G_02785 | Site-specific tyrosine recombinase XerD | ns | ns | -3.23 | 0 (0.0001) | -2.63 | 2 (0.0018) | -2.22 | 3 (0.0007) | -1.79 | 8 (0.0052) | ||
A1G_05315 | Chaperonin GroEL | ns | ns | -3.45 | 0 (0.0001) | -2.04 | 9 (0.0115) | -2.78 | 6 (0.0021) | -2.44 | 0 (0.0002) | ||
A1G_07375 | DNA-damage-inducible protein J | ns | ns | -3.13 | 0 (0.0001) | ns | -2.56 | 3 (0.0007) | ns | ||||
A1G_07470 | DNA mismatch repair protein | ns | ns | 1.64 | 87 (0.0006) | 1.29 | 76 (0.0332) | ns | ns | ||||
A1G_00295 | Cell surface antigen-like protein Sca10 | -2.33 | 3 (0.0031) | -2.22 | 9 (0.0123) | -3.45 | 0 (0.0001) | -2.44 | 0 (0.0009) | -8.33 | 0 (0.0002) | -5.56 | 0 (0.0002) |
A1G_01440 | Cell surface antigen-like protein Sca8 | ns | ns | -4.35 | 0 (0.0001) | -2.94 | 0 (0.0009) | -4.00 | 0 (0.0002) | -3.70 | 0 (0.0002) | ||
A1G_06915 | Cell surface antigen-like protein Sca13 | ns | ns | -3.33 | 9 (0.0047) | ns | ns | ns | |||||
A1G_03790 | Cell surface antigen | -1.89 | 3 (0.0031) | -2.50 | 5 (0.0034) | ns | ns | -2.70 | 0 (0.0002) | -2.50 | 5 (0.0017) | ||
A1G_06030 | Outer membrane protein B (cell surface antigen sca5) | ns | ns | ns | ns | 1.59 | 75 (0.0242) | ns | |||||
A1G_02865 | Lysyl-tRNA synthetase | 1.80 | 93 (0.0050) | 2.02 | 92 (0.0099) | ns | ns | 1.55 | 78 (0.0122) | ns | |||
A1G_03300 | Leucyl-tRNA synthetase | 4.28 | 96 (0.0036) | 2.04 | 85 (0.0432) | ns | ns | 4.51 | 90 (0.0002) | 2.02 | 76 (0.0277) | ||
RrIowa_1080 | Arp2/3 complex activation protein | -1.92 | 10 (0.0137) | ns | -2.17 | 0 (0.0001) | -2.22 | 2 (0.0018) | -4.17 | 0 (0.0002) | -3.03 | 5 (0.0017) | |