Leishmania infantum-specific production of IFN-γ and IL-10 in stimulated blood from dogs with clinical leishmaniosis

Table 3 Summary of parameters based on IFN-γ producer versus IFN-γ non-producer dogs

Clinical staging	IFN-γ producers (mean ± standard deviation)					IFN-γ non-producers (mean ± standard deviation)
Clinical staging	Number of dogs	IFN-γ LSA (pg/ml)	IL-10 LSA (pg/ml)	PCR (parasite/ml)	ELISA (EU)	Number of dogs	IFN-γ LSA (pg/ml)	IL-10 LSA (pg/ml)	PCR (parasite/ml)	ELISA (EU)
I	9	2247 ± 2749	18 ± 20	4.1 ± 11	50 ± 53	1	0 ± 0	15 ± 0	0 ± 0	13 ± 0
IIa	17	3031 ± 5240	12 ± 16	2 ± 4	2660 ± 7140	10	0 ± 0	3 ± 8	12 ± 14	10,418 ± 15,420
IIb	3	1616 ± 1860	14 ± 23	0.1 ± 0.1	14,373 ± 15,473	6	0 ± 0	3.3 ± 8	162 ± 360	27,744 ± 32,476
III	5	846 ± 665	29 ± 18	12 ± 19	7755 ± 5590	5	0 ± 0	0 ± 0	29 ± 31	17,077 ± 28,897
IV	1	375 ± 0	19 ± 0	35 ± 0	73,701 ± 0	3	0 ± 0	0 ± 0	8438 ± 14,419	44,967 ± 51,408
Total	35	2320 ± 3961^a	16 ± 18^b	5 ± 10	5750 ± 14,082	25	0 ± 0	2.4 ± 7	1100 ± 5112^c	19,638 ± 28,596^d

Statistical study was performed to compare IFN-γ producers versus non-IFN-γ producers. Letters a to d correspond to statistically significant differences
Results for IFN-γ LSA, IFN-γ^a producers > non-IFN-γ producers (Mann-Whitney U-test: Z = -6.7, P < 0.0001)
Results for IL-10 LSA, IFN-γ^b producers > non-IFN-γ producers (Mann-Whitney U-test: Z = -3.60, P < 0.0001)
Results for PCR, IFN-γ^c producers< non-IFN-γ producers (Mann-Whitney U-test: Z = -3.2, P = 0.001)
Results for ELISA units, IFN-γ^d producers< non-IFN-γ producers (Mann-Whitney U-test: Z = -3.2, P = 0.001)
Abbreviations: LSA L. infantum soluble antigen

ISSN: 1756-3305