Fig. 4

Immunolocalisation of SjAChE in adult S. japonicum and four-day mechanically transformed schistosomula. Adult male a and female b worm sections were labelled with rabbit anti-SjAChE antibody coupled with anti-rabbit HRP and scanned using an Aperio scanner. c Negative control sections of female worm were incubated with rabbit pre-immune serum. The female gut (b, c) appears non-specifically opaque due to the presence of red blood cell products, with the histochemical negative control demonstrating the dark region is due to gut contents. d Immunofluorescence of SjAChE in four-day old schistosomula probed with rabbit anti-SjAChE antibody. e Brightfield and corresponding fluorescence images f of schistosomula negative controls using pre-immune rabbit serum. Donkey anti-rabbit IgG 555 was used as secondary antibody and positive immunofluorescence is shown in red. As a negative control, f shows there is no signal produced when incubating parasites with rabbit pre-immune serum in the adult worm tissue. Scale-bars: a-c, 100 μm; d-f, 20 μm