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Fig. 7 | Parasites & Vectors

Fig. 7

From: The tandemly repeated NTPase (NTPDase) from Neospora caninum is a canonical dense granule protein whose RNA expression, protein secretion and phosphorylation coincides with the tachyzoite egress

Fig. 7

Phosphorylation detection of NcGRA7 and NcNTPase by Phos-Tag 1-DE. Tachyzoite extracts obtained at 56 hpi were processed with alkaline phosphatase (CIP) and phosphatase inhibitors (PI), electrophoresed on 1-DE and Phos-Tag 1-DE gels, and blotted to nitrocellulose membranes. Then, both proteins were detected by their respective antibodies in order to detect a mobility shift of the proteins treated with PI

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Parasites & Vectors

ISSN: 1756-3305

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