Fig. 2

Phylogenetic analysis of ITS-1 sequences obtained from RMF-positive blood samples from cervid hosts. Sequences of 609 base pairs were aligned using ClustalW, and the evolutionary history was inferred using the Neighbor-Joining method. Evolutionary distances were computed using the Kimura 2-parameter method. The tree is drawn to scale. Bootstrap values (×1000) greater than 50 % are shown above the branches. RMF isolates are marked with solid boxes; ITS-1 Clades A and B are labelled. RA-F3 (Rumenfilaria andersoni; open box), Setaria yehi, and Elaeophora schneideri serve as reference standards. GenBank accession numbers for all isolates are listed in Tables 1 and 3