Isolation, cultivation and molecular characterization of a new Trypanosoma equiperdum strain in Mongolia

Parasites & Vectors

Table 2 Summary of the maxicircle PCR results

Target locus^a	T. b. brucei	T. evansi	T. equiperdum
Target locus^a	GUTat3.1	IL3960	IVM-t1	STIB818	STIB841	STIB842	BoTat1.1
NAD7	P	N	N	N	P	P	P
Cox2	P	N	N	N	P	P	P
A6	P	N	N	N	P	P	P
12S rRNA	P	N	N	P	P	P	P
ND7-CyB	P	N	N	N	P	P	P
MURF1-ND1	P	N	N	N	P	P	P
MURF2-Cox1	P	N	N	N	P	P	P
ND4-ND5	P	N	P	P	P	P	P

All of the PCR product sequences were confirmed by a sequence analysis and the NCBI BlastN software program
Abbreviations: P Positive, N Negative
^aNAD7, NADH-dehydrogenase subunit 7; Cox2, Cytochrome oxidase subunit 2; A6, ATPase subunit 6; 12S rRNA, 12S ribosomal RNA; ND7-CyB, NADH-dehydrogenase subunit 7-cytochrome b; MURF-ND1, Maxicircle unknown reading frame-NADH dehydrogenase subunit 1; MURF2-Cox1, Maxicircle unknown reading frame 2-cytochrome oxidase subunit 1 and ND4-ND5, NADH-dehydrogenase subunits 4–5

ISSN: 1756-3305