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Table 3 Development of East Coast fever symptoms in cattle vaccinated with the multivalent antigen cocktail after exposure to Theileria parva-infected ‘low-line’ ticks

From: Induction of humoral immune response to multiple recombinant Rhipicephalus appendiculatus antigens and their effect on tick feeding success and pathogen transmission

  

Vaccinated cattle (n = 18)

Control cattle (n = 10)

P-value

Pyrexiaa,b

Number of animals where symptom observed (%)

15 (83 %)

9 (90 %)

< 0.999d

Day of first onset

10.3 (1.2)

11.1 (1.8)

0.801e

Duration

5.8 (0.9)

5. 6 (1.4)

0.803e

Regional lymph node parasitosisa

Number of animals where symptom observed (%)

13 (72 %)

9 (90 %)

0.375d

Day of first onset

13.5 (0.6)

14.0 (1.1)

0.774e

Duration

4.2 (0.7)

3.7 (0.9)

0.547e

Contra-lateral lymph node parasitosisa

Number of animals developing symptom (%)

8 (44 %)

6 (60 %)

0.695d

Day of first onset

16.1 (0.5)

17.2 (0.6)

0.211e

Duration

3 (0.8)

2.5 (1.7)

0.825e

Piroplasma

Number of animals where symptom observed (%)

5 (28 %)

4 (40 %)

0.678d

Day of first onset

17.6 (0.6)

17.0 (0.4)

0.722e

Duration

3 (0.6)

3 (0.7)

< 0.999e

Nested p104 PCR detection of parasites

 

17 (94 %)

9 (90 %)

< 0.999d

PIM antibodies

Number of animals where antibodies detected (%)

18 (100 %)

10 (100 %)

< 0.999d

Average PP valuec

44.7 ± 16.1

53.4 ± 22.8

0.249e

  1. aValues are displayed as the average day of first detection (SEM). Where symptoms were not observed in an animal, no values are reflected
  2. bPyrexia was defined as rectal temperature exceeding 39.5 °C
  3. cThe average Percentage Positive (PP) value calculated as the (OD of test sample/OD of strong positive) × 100
  4. dFischer’s exact test
  5. eMann-Whitney test