Fig. 4

Specific expression of Ha24 in Hyalomma asiaticum. a Analysis of specific expression of Ha24 gene according to the sex. Lanes 1–4, 5–8: the cDNA of unfed male adult ticks, unfed female adult ticks, fed male adult ticks and partially fed female adult ticks (Lanes 1–4: actin primers; Lanes 5–8: Ha24 gene specific primers). b Expression of Ha24 in different development periods. Lanes 1–3, 4–6: the cDNA of eggs, larvae, and nymphs (Lanes 1–3: actin primers; Lanes 4–6: Ha24 gene specific primers). c Expression of Ha24 in the salivary glands and midguts of H. asiaticum. Lanes 1–2, salivary glands and midguts with actin primers, respectively; Lanes 3–4, salivary glands and midguts with Ha24 gene specific primers, respectively