Table 1 Primers used for rapid amplification of cDNA ends, RT-qPCR and dsRNA
From: Role of NF-kβ factor Rel2 during Plasmodium falciparum and bacterial infection in Anopheles dirus
Primer | Sequence 5′-3′ |
|---|---|
RACE 5′ Rel2 GSP | CGCTTCTGCGGGTCCACCTGGTACAGGGG |
RACE 5′ Rel2 NGSP | CAGCGAGCCGTGCGTACCGTGCATCTCC |
RACE 3′ Rel2 GSP | GCACGGTACGCACGGCTCGCTGATGGGC |
RACE 3′ Rel2 NGSP | CGCCGGTGAGGCTAAGGTGCGCTGCTCC |
RT-qPCR S7-F | AGAAGAAGTTCTCCGGTAAG |
RT-qPCR S7-R | CGGTCTCTTCTGCTTGT |
RT-qPCR Rel2-F | GGCAGGACAATCTACAAAC |
RT-qPCR Rel2-R | CTCCAGGATCACGAGATAG |
dsRel2-F | CCCTACTGAATTTGGACAGC |
dsRel2-R | ATTTGCTGCTGTTGGGACTG |
dsLacZ-F | CATTACCAGGCCGAAGCAG |
dsLacZ-R | GCGGCTGATGTTGAACTGGAAG |