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Table 2 Microbiological parameters and physical parameters determined for pasteurized and unpasteurized harvested rainwater samples

From: Molecular detection of Acanthamoeba spp., Naegleria fowleri and Vermamoeba (Hartmannella) vermiformis as vectors for Legionella spp. in untreated and solar pasteurized harvested rainwater

Date Unpasteurized and pasteurized sample temp. (°C) pH Heterotrophic plate count (CFU/ml) No. of Legionella isolates obtained Gene copies/ml
Legionella spp. Acanthamoeba spp. Vermamoeba vermiformis Naegleria fowleri
22.10.2015 24 8.4 1.5 × 106 9 6.5 × 104 9.8 × 104 5.7 × 106 1.6 × 105
68a 8.4 BDLb BDLb 3.2 × 103 8.4 × 103 9.4 × 103 LLODd
22.10.2015 25 8.1 1.0 × 106 2 4.5 × 104 8.3 × 104 3.9 × 104 6.4 × 104
74a 8.5 BDLb BDLb 9.2 × 103 5.2 × 103 LLODc LLODd
19.10.2015 21 8.3 6.8 × 105 1 5.7 × 106 1.3 × 105 1.4 × 105 9.2 × 104
84a 8.2 BDLb BDLb 2.3 × 103 1.7 × 104 LLODc LLODd
27.10.2015 31 8.4 2.7 × 105 3 8.2 × 106 6.5 × 104 3.2 × 105 1.0 × 106
93a 8.2 BDLb BDLb 1.1 × 103 1.4 × 104 LLODc LLODd
  1. aPasteurized rainwater sample
  2. b BDL below detection limit
  3. c LLOD lower limit of detection: Vermamoeba vermiformis (< 5–8 gene copies/μl)
  4. d LLOD lower limit of detection: Naegleria fowleri (< 12–17 gene copies/μl)