Molecular detection of Acanthamoeba spp., Naegleria fowleri and Vermamoeba (Hartmannella) vermiformis as vectors for Legionella spp. in untreated and solar pasteurized harvested rainwater

Table 2 Microbiological parameters and physical parameters determined for pasteurized and unpasteurized harvested rainwater samples

Date	Unpasteurized and pasteurized sample temp. (°C)	pH	Heterotrophic plate count (CFU/ml)	No. of Legionella isolates obtained	Gene copies/ml
Date	Unpasteurized and pasteurized sample temp. (°C)	pH	Heterotrophic plate count (CFU/ml)	No. of Legionella isolates obtained	Legionella spp.	Acanthamoeba spp.	Vermamoeba vermiformis	Naegleria fowleri
22.10.2015	24	8.4	1.5 × 10⁶	9	6.5 × 10⁴	9.8 × 10⁴	5.7 × 10⁶	1.6 × 10⁵
22.10.2015	68^a	8.4	BDL^b	BDL^b	3.2 × 10³	8.4 × 10³	9.4 × 10³	LLOD^d
22.10.2015	25	8.1	1.0 × 10⁶	2	4.5 × 10⁴	8.3 × 10⁴	3.9 × 10⁴	6.4 × 10⁴
22.10.2015	74^a	8.5	BDL^b	BDL^b	9.2 × 10³	5.2 × 10³	LLOD^c	LLOD^d
19.10.2015	21	8.3	6.8 × 10⁵	1	5.7 × 10⁶	1.3 × 10⁵	1.4 × 10⁵	9.2 × 10⁴
19.10.2015	84^a	8.2	BDL^b	BDL^b	2.3 × 10³	1.7 × 10⁴	LLOD^c	LLOD^d
27.10.2015	31	8.4	2.7 × 10⁵	3	8.2 × 10⁶	6.5 × 10⁴	3.2 × 10⁵	1.0 × 10⁶
27.10.2015	93^a	8.2	BDL^b	BDL^b	1.1 × 10³	1.4 × 10⁴	LLOD^c	LLOD^d

^aPasteurized rainwater sample
^b BDL below detection limit
^c LLOD lower limit of detection: Vermamoeba vermiformis (< 5–8 gene copies/μl)
^d LLOD lower limit of detection: Naegleria fowleri (< 12–17 gene copies/μl)

ISSN: 1756-3305