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Table 3 The lower limit of detection (LLOD), amplification efficiency, correlation coefficient (r 2), intra- and inter-assay reducibility within the range of 109 to 101 gene copies/μl of each qPCR assay

From: Molecular detection of Acanthamoeba spp., Naegleria fowleri and Vermamoeba (Hartmannella) vermiformis as vectors for Legionella spp. in untreated and solar pasteurized harvested rainwater

Organisms assayed LLOD (gene copies/μl) Amplification efficiency (%) Correlation coefficient (r 2) Mean ± SD of CV
Intra-assay Inter-assay
Legionella spp. 8–12 1.86 (93)–1.94 (97) 0.99–1.00 0.160 ± 0.257 0.251 ± 0.220
Acanthamoeba spp. 5–11 1.92 (96)–1.95 (98) 0.99–1.00 0.142 ± 0.283 0.192 ± 0.225
Vermamoeba vermiformis 5–8 1.85 (93)–1.89 (95) 0.98–1.00 0.09 ± 0.112 0.129 ± 0.092
Naegleria fowleri 12–17 1.90 (95)–2.04 (102) 0.99–1.00 0.195 ± 0.410 0.348 ± 0.251
  1. Abbreviations: CV coefficient of variation, LLOD lower limit of detection, SD standard deviation