Table 3 The lower limit of detection (LLOD), amplification efficiency, correlation coefficient (r 2), intra- and inter-assay reducibility within the range of 109 to 101 gene copies/μl of each qPCR assay
Organisms assayed | LLOD (gene copies/μl) | Amplification efficiency (%) | Correlation coefficient (r 2) | Mean ± SD of CV | |
|---|---|---|---|---|---|
Intra-assay | Inter-assay | ||||
Legionella spp. | 8–12 | 1.86 (93)–1.94 (97) | 0.99–1.00 | 0.160 ± 0.257 | 0.251 ± 0.220 |
Acanthamoeba spp. | 5–11 | 1.92 (96)–1.95 (98) | 0.99–1.00 | 0.142 ± 0.283 | 0.192 ± 0.225 |
Vermamoeba vermiformis | 5–8 | 1.85 (93)–1.89 (95) | 0.98–1.00 | 0.09 ± 0.112 | 0.129 ± 0.092 |
Naegleria fowleri | 12–17 | 1.90 (95)–2.04 (102) | 0.99–1.00 | 0.195 ± 0.410 | 0.348 ± 0.251 |