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Fig. 1 | Parasites & Vectors

Fig. 1

From: Discrimination between Onchocerca volvulus and O. ochengi filarial larvae in Simulium damnosum (s.l.) and their distribution throughout central Ghana using a versatile high-resolution speciation assay

Fig. 1

Genetic discrimination of O. volvulus and O. ochengi. a Sequence of the PCR 79-bp amplicon used to discriminate between O. volvulus and O. ochengi. Nucleotide differences between the two species are highlighted in bold, and the ApaI restriction site present in the O. volvulus sequence is underlined. b Normalised HRM melt curves from cloned positive control 79-bp products (pGem_Ov and pGem_Oo), adult O. volvulus and O. ochengi DNA, and 4 larvae samples from both species, each in duplicate. Curve colour of the larval samples is automatically determined based on the clustering of melt curves to the O. volvulus (green) and O. ochengi (red) adult samples. c The same samples are presented as in (b), showing HRM difference curves, which accentuate differences between the melt curve clusters in (b), normalised to the O. ochengi cluster. d Representative RFLP analysis of amplicons generated in the HRM assay for each species, showing digestion of the O. volvulus sequence, but not the O. ochengi sequence, with ApaI

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Parasites & Vectors

ISSN: 1756-3305

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