Fig. 5

Tregs and Th17 cell differentiation and cytokines production in splenocytes of mice immunized with rTsPmy. Splenocytes were isolated from mice immunized with rTsPmy or Sf9 protein and sorted for CD4, CD25, Foxp3 and Th17 by FACS (a, b). The different cytokines in splenocyte culture were determined by ELISA (c). Data (means ± standard deviations) are representative of three independent experiments. *P < 0.05; **P <0.01; ***P <0.001 compared to PBS or Sf9 control as indicated