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Fig. 5 | Parasites & Vectors

Fig. 5

From: Identification of interchangeable cross-species function of elongation factor-1 alpha promoters in Babesia bigemina and Babesia bovis

Fig. 5

a Heterologous function of the B. bigemina IG promoters. Functional promoter analysis of the B. bigemina IG regions in B. bovis transfected parasites. The Y axis represents the PPE, and the Z axis represents RLU. The X axis represents the different plasmid constructs (Ig-A, Ig-A Rev, Ig-B, Ig-B Rev, 4-35-ef-luc, Pr-, pBluescript) transfected and the control (4-35-ef-luc not transfected) at the time of the measurements performed at different hours post-electroporation. The black bars represent the PPE ± standard deviation (SD). The grey line represents relative luciferase units (RLU). b Schematic representation of the plasmid constructs used in the transient transfections and the RLU obtained 24 h after electroporation. Asterisks represent statistically significant differences detected using the t- test (P < 0.05)

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