GP50 as a promising early diagnostic antigen for Taenia multiceps infection in goats by indirect ELISA

Parasites & Vectors

Table 1 Determination of the optimal protein coating concentration and serum dilution for indirect ELISA

Antisera at different dilutions	OD₄₅₀ values of antigen at different coating concentrations
Antisera at different dilutions	0.098 μg	0.197 μg	0.394 μg	0.787 μg	1.574 μg	3.148 μg
1:5 (P)	1.043	1.104	1.116	1.160	1.216	1.274
1:5 (N)	0.417	0.442	0.551	0.720	1.035	1.036
1:10 (P)	0.807	0.921	0.888	0.877	0.952	0.973
1:10 (N)	0.307	0.333	0.440	0.607	0.753	0.771
1:20 (P)	0.584	0.626	0.616	0.741	0.853	0.926
1:20 (N)	0.255	0.264	0.300	0.425	0.594	0.605
1:40 (P)	0.427	0.429	0.506	0.507	0.592	0.645
1:40 (N)	0.200	0.243	0.260	0.332	0.468	0.527
1:80 (P)	0.367	0.393	0.398	0.405	0.392	0.581
1:80 (N)	0.187	0.203	0.215	0.242	0.244	0.337

Abbreviations: N positive serum, P negative serum
Note: Figures in bold represent the optimum conditions for this indirect ELISA method

ISSN: 1756-3305