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Table 3 Toxicity of 23 Bt isolates and the Bti-H14 reference strain against third-instar larval stage of An. gambiae 24 h post-treatment

From: Isolation and characterization of native Bacillus thuringiensis strains from Saudi Arabia with enhanced larvicidal toxicity against the mosquito vector Anopheles gambiae (s.l.)

Bt-code Region LC50 (μg/ml) a LC95 (μg/ml) a Slope ± SE CFU/μg × 105 b
Bti-H14 13.3 (11.2–15.9) 63.7 (40.4–102.3) 2.4 ± 0.07 4.5 ± 0.3
Bt05 Kharj 13.4 (11.07–16.5) 78.5 (46.0–137.0) 2.15 ± 0.06 1.0 ± 0.1
Bt07 Asseer 15.0 (12.2–18.8) 90.4 (50.5–166.0) 2.12 ± 0.07 1.0 ± 0.2
Bt10 Qassim 5.0 (4.4–5.6)* 18.6 (14.6–23.8) 2.90 ± 0.06 1.2 ± 0.3
Bt11 Yanbu 12.3 (10.2–14.8)* 72.6 (43.5–124.3) 2.10 ± 0.06 0.65 ± 0.3
Bt12 Yanbu 9.5 (8.3–10.9)* 41.6 (29.4–59.6) 2.60 ± 0.06 0.53 ± 0.4
Bt16 Asser 13.0 (10.6–16.0) 84.2 (47.8–152.6) 2.02 ± 0.06 0.92 ± 0.3
Bt17 Hafr-elbaten 22.0 (18.2–26.7) 128.5 (76.4–219.7) 2.14 ± 0.06 8.1 ± 0.4
Bt26 Madinah 12.9 (9.9–16.9) 149.7 (64.7–367.2) 1.54 ± 0.05 4.1 ± 0.7
Bt27 Madinah 7.1 (6.1–8.2)* 40.1 (27.3–60.2) 2.20 ± 0.05 5.9 ± 0.4
Bt28 Makkah 8.6 (7.7–9.6)* 27.5 (22.4–33.9) 3.30 ± 0.07 6.2 ± 0.3
Bt29 Madinah 14.4 (12.4–16.7) 49.4 (34.1–72.3) 3.10 ± 0.12 0.52 ± 0.1
Bt42 Jezan 5.9 (5.2–6.8)* 24.3 (18.9–31.3) 2.70 ± 0.07 0.93 ± 0.3
Bt44 Jezan 16.1 (13.7–18.9) 91.9 (59.3–144.2) 2.20 ± 0.05 0.63 ± 0.4
Bt53 Madinah 13.8 (11.7–16.4) 101.7 (61.3–171.9) 1.90 ± 0.05 1.3 ± 0.4
Bt55 Madinah 4.1 (3.6–4.6)* 14.4 (11.6–18.1) 3.02 ± 0.07 1.95 ± 0.3
Bt56 Madinah 11.7 (10.2–13.5)* 43.8 (31.2–62.2) 2.90 ± 0.08 0.15 ± 0.3
Bt57 Madinah 7.4 (6.5–8.25)* 26.1 (20.9–32.9) 2.98 ± 0.07 1.27 ± 0.3
Bt58 Madinah 5.7 (5.1–6.5)* 24.6 (18.6–32.9) 2.60 ± 0.05 1.24 ± 0.2
Bt59 Madinah 10.3 (8.5–12.6)* 84.9 (46.3–161.6) 1.80 ± 0.05 1.28 ± 0.2
Bt60 Madinah 14.7 (12.5–17.5) 58.2 (38.1–89.9) 2.75 ± 0.10 0.13 ± 0.1
Bt63 Makkah 3.9 (3.4–4.4)* 15.7 (12.3–20.3) 2.70 ± 0.06 0.7 ± 0.2
Bt67 Madinah 6.9 (5.9–8.2)* 50.6 (31.5–83.4) 1.90 ± 0.05 0.6 ± 0.1
Bt68 Madinah 5.2 (4.5–5.8)* 21.6 (16.6–28.5) 2.64 ± 0.06 1.05 ± 0.3
  1. *Asterisks indicate significant differences compared to the reference Bti-H14
  2. aLC50 and LC95, Lethal concentration that kills 50 and 95% of larval population (n = 20), respectively (calculated by Probit analysis and reported with 95% confidence intervals)
  3. bCFU, colony forming unit (mean ± standard error). Each bioassay comprised five concentrations, one replicate for each concentration, and were repeated five times (n = 5)