Fig. 7

AB-PAS stain of mucins in the intestinal epithelium of oral administration mice. Jejunum tissue sections of each group were collected, fixed, and stained with AB-PAS. Acid mucins were dyed to blue, neutral mucin were dyed red, and the alkaline and neutral mixed mucins were dyed amaranth. Panels a-b, c-d, e-f and g-h indicate PBS, B.s-CotC, BL21-CsCP and B.s-CotC-CsCP orally administered groups at week 4, respectively. Panels (i) and (j) show the B.s-CotC-CsCP group treated at week 6. Scale-bars: a, c, e, g, i, 200 μm; b, d, f, h, j, 50 μm. The arrows indicate acidic mucins secreted by goblet cells