Fig. 2

a Western blot analysis of purified rPSOP25 protein and P. berghei ookinete (Ook) lysates with anti-rPSOP25 sera (PolyAb) and anti-rPSOP25 mAb. Lysates were subjected to electrophoresis under reducing conditions by SDS-PAGE. Pbs21 mAb was used as positive control; a control mouse serum (PBS) was used as negative control. b IFA was performed on zygote, retort and ookinete at different time points of ookinete culture using anti-rPSOP25 mAb (green - FITC). Positive control - Pbs21 mAb, negative control - a control mouse serum (PBS). Nuclei were labelled with DAPI (blue). BF, bright field. Scale-bars: 5 μm