Fig. 4

a Schematic representation of the WT locus, the construct used for transfection and the recombined locus with psop25 replaced with the hdhfr cassette. Primers QCR1, QCR2, GW2, hdhfr1, hdhfr2, GW1 and GT used for diagnostic PCR of the WT locus or knockout are marked. b Lane 1: primers QCR1 + QCR2 (696 bp) are used for diagnostic PCR of the WT locus. Lanes 2, 3 and 4 are PCR products from primers GW2 + QCR2 (1,002 bp), hdhfr1 + hdhfr2 (561 bp), GW1 + GT (1,831 bp) for PCR verification of psop25 KO, respectively