Genetic clustering and polymorphism of the merozoite surface protein-3 of Plasmodium knowlesi clinical isolates from Peninsular Malaysia

Table 1 Estimates of DNA diversity, selection, and neutrality tests of full length, Domain A and Domain B of pkmsp3 gene

Pkmsp3	Sites^a	Ss	S	Ps	Hd ± SD	π ± SD	d_N ± SE	d_S ± SE	d_N/d_S	Z-test P-values			Tajima’s D	Fu & Li’s
Pkmsp3	Sites^a	Ss	S	Ps	Hd ± SD	π ± SD	d_N ± SE	d_S ± SE	d_N/d_S	d_N = d_S	d_N > d_S	d_N < d_S	Tajima’s D	D*	F*
Full length	1,077	384	64	320	0.997 ± 0.005	0.046 ± 0.011	0.052 ± 0.004	0.058 ± 0.008	1.1	0.45	1.00	0.24	-1.440	-0.077	1.046
Domain A	534	104	44	60	0.989 ± 0.007	0.039 ± 0.002	0.039 ± 0.002	0.030 ± 0.008	1.3	0.34	0.10	1.00	-0.723	-1.852	-1.670
Domain B	483	273	16	257	0.989 ± 0.007	0.067 ± 0.025	0.025 ± 0.007	0.042 ± 0.002	0.6	0.19	1.00	0.09	-1.918	-1.579	-1.711

Abbreviations: d _N non-synonymous polymorphism, d _S synonymous polymorphism, d _N /d _S ratio of d_N to d_S, Hd haplotype diversity, Ps number of informative-parsimonious sites, π nucleotide diversity, S number of singleton sites, SD standard deviation, Ss number of segregating sites
^aTotal number of sites analysed excluding gaps
*Modified Fu & Li’s D and F tests

ISSN: 1756-3305