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Table 1 Reconfirmation of the potential positive interactions using small-scale mating

From: Identification of host proteins interacting with Toxoplasma gondii GRA15 (TgGRA15) by yeast two-hybrid system

Y2HGold yeast strain Y187 yeast strain
Prey1 Prey2 Prey3 Prey4 Prey5 Prey6 Prey7 Prey8
Empty pGBKT7 vector C1 C2 C3 C4 C5 C6 C7 C8
pGBKT7-GRA15 bait plasmid 1 2 3 4 5 6 7 8
  1. Prey1–8: Prey plasmid isolated from eight blue clones
  2. Prey1–8 were used to transform Y187 cells, respectively, followed by each transformant mating with Y2HGold containing pGBKT7 empty vector to create zygotes (C1-C8)
  3. Prey1–8 were used to transform Y187 cells, respectively, followed by each transformant mating with Y2HGold containing pGBKT7-GRA15 bait plasmid to create zygotes (18)