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Table 1 Reconfirmation of the potential positive interactions using small-scale mating

From: Identification of host proteins interacting with Toxoplasma gondii GRA15 (TgGRA15) by yeast two-hybrid system

Y2HGold yeast strain

Y187 yeast strain

Prey1

Prey2

Prey3

Prey4

Prey5

Prey6

Prey7

Prey8

Empty pGBKT7 vector

C1

C2

C3

C4

C5

C6

C7

C8

pGBKT7-GRA15 bait plasmid

1

2

3

4

5

6

7

8

  1. Prey1–8: Prey plasmid isolated from eight blue clones
  2. Prey1–8 were used to transform Y187 cells, respectively, followed by each transformant mating with Y2HGold containing pGBKT7 empty vector to create zygotes (C1-C8)
  3. Prey1–8 were used to transform Y187 cells, respectively, followed by each transformant mating with Y2HGold containing pGBKT7-GRA15 bait plasmid to create zygotes (18)
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Parasites & Vectors

ISSN: 1756-3305

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