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Fig. 4 | Parasites & Vectors

Fig. 4

From: A next-generation microarray further reveals stage-enriched gene expression pattern in the blood fluke Schistosoma japonicum

Fig. 4

qPCR validation of stage-enriched genes. The expression of 5 selected genes enriched in cercariae (a), hepatic schistosomula (b), adult worms (c), and eggs (d), respectively, was quantified by qRT-PCR analysis. The PSMD4 gene was used for internal normalization among the four developmental stages. The highest expression level in one particular stage was set as 1. The error bars represent the standard deviation for three technical replicates. Correlations between the microarray and qPCR results for the selected genes enriched in cercariae (e), hepatic schistosomula (f), adult worms (g), and eggs (h), were analysed using Spearman’s rho

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