Fig. 3

Antibody isotypes in sera of Wuchereria bancrofti-exposed humans to recombinant proteins. IgA, IgG1, IgG2, IgG3, IgG4, IgE and IgM isotype antibodies in various groups (EN, MF, CP and NEN) for a Bm-TPP; b BmAF-Myo; c Wol Tl IF-1; and d wBm-LigA proteins, measured by ELISA after coating the plate with 0.1 μg/ml of each protein antigen. Data (OD values corresponding to antibody isotype reactivity of serum of all individuals of a group for each protein) are summarized as mean + standard deviation (NEN, n = 10; EN, MF and CP, n = 20). For each protein, comparison between groups was performed by Kruskal-Wallis ANOVA and the significance of the differences in mean ranks between groups was assessed by Mann-Whitney U test. Data are from one of the three similar experiments using same serum sample. ^* P < 0.05; ^** P < 0.01; ^*** P < 0.001